The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons.

Kim JY, Oh MH, Bernard LP, Macara IG, Zhang H
J Biol Chem. 2011 286 (43): 37615-24

PMID: 21900250 · PMCID: PMC3199506 · DOI:10.1074/jbc.M111.268029

Dendritic spines are actin-rich structures, the formation and plasticity of which are regulated by the Rho GTPases in response to synaptic input. Although several guanine nucleotide exchange factors (GEFs) have been implicated in spine development and plasticity in hippocampal neurons, it is not known how many different Rho GEFs contribute to spine morphogenesis or how they coordinate the initiation, establishment, and maintenance of spines. In this study, we screened 70 rat Rho GEFs in cultured hippocampal neurons by RNA interference and identified a number of candidates that affected spine morphogenesis. Of these, Dock180, which plays a pivotal role in a variety of cellular processes including cell migration and phagocytosis, was further investigated. We show that depletion of Dock180 inhibits spine morphogenesis, whereas overexpression of Dock180 promotes spine morphogenesis. ELMO1, a protein necessary for in vivo functions of Dock180, functions in a complex with Dock180 in spine morphogenesis through activating the Rac GTPase. Moreover, RhoG, which functions upstream of the ELMO1/Dock180 complex, is also important for spine formation. Together, our findings uncover a role for the RhoG/ELMO1/Dock180 signaling module in spine morphogenesis in hippocampal neurons.

MeSH Terms (13)

Adaptor Proteins, Signal Transducing Animals Cell Movement GTP Phosphohydrolases Hippocampus Morphogenesis Nerve Tissue Proteins Neurons rac GTP-Binding Proteins Rats RNA Interference Signal Transduction Spine

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