Genetic engineering of human pluripotent cells using TALE nucleases.

Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
Nat Biotechnol. 2011 29 (8): 731-4

PMID: 21738127 · PMCID: PMC3152587 · DOI:10.1038/nbt.1927

Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).

MeSH Terms (13)

Base Sequence Embryonic Stem Cells Endonucleases Gene Targeting Genetic Engineering Homeodomain Proteins Humans Induced Pluripotent Stem Cells Molecular Sequence Data Myosin-Light-Chain Phosphatase Octamer Transcription Factor-3 Transcription Factors Zinc Fingers

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