Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription.

O'Brien RM, Lucas PC, Forest CD, Magnuson MA, Granner DK
Science. 1990 249 (4968): 533-7

PMID: 2166335 · DOI:10.1126/science.2166335

Phosphoenolpyruvate carboxykinase (PEPCK) governs the rate-limiting step in gluconeogenesis. Glucocorticoids and adenosine 3',5'-monophosphate (cAMP) increase PEPCK gene transcription and gluconeogenesis, whereas insulin has the opposite effect. Insulin is dominant, since it prevents cAMP and glucocorticoid-stimulated transcription. Glucocorticoid and cAMP response elements have been located in the PEPCK gene and now a 15-base pair insulin-responsive sequence (IRS) is described. Evidence for a binding activity that recognizes this sequence is presented.

MeSH Terms (15)

Animals Base Sequence Cell Line Chloramphenicol O-Acetyltransferase Cyclic AMP Dexamethasone Genes, Regulator Insulin Molecular Sequence Data Phosphoenolpyruvate Carboxykinase (GTP) Recombinant Fusion Proteins RNA, Messenger Thionucleotides Transcription, Genetic Transfection

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