Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy.

Peckys DB, de Jonge N
Nano Lett. 2011 11 (4): 1733-8

PMID: 21410218 · PMCID: PMC3087289 · DOI:10.1021/nl200285r

The intracellular uptake of 30 nm diameter gold nanoparticles (Au-NPs) was studied at the nanoscale in pristine eukaryotic cells. Live COS-7 cells were maintained in a microfluidic chamber and imaged using scanning transmission electron microscopy. A quantitative image analysis showed that Au-NPs bound to the membranes of vesicles, possibly lysosomes, and occupied 67% of the available surface area. The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles.

MeSH Terms (10)

Animals Cell Membrane Chlorocebus aethiops COS Cells Diffusion Gold Materials Testing Microscopy, Electron, Scanning Transmission Nanostructures Solutions

Connections (1)

This publication is referenced by other Labnodes entities: