Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy.

Peckys DB, de Jonge N
Nano Lett. 2011 11 (4): 1733-8

PMID: 21410218 · PMCID: PMC3087289 · DOI:10.1021/nl200285r

The intracellular uptake of 30 nm diameter gold nanoparticles (Au-NPs) was studied at the nanoscale in pristine eukaryotic cells. Live COS-7 cells were maintained in a microfluidic chamber and imaged using scanning transmission electron microscopy. A quantitative image analysis showed that Au-NPs bound to the membranes of vesicles, possibly lysosomes, and occupied 67% of the available surface area. The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles.

MeSH Terms (10)

Animals Cell Membrane Cercopithecus aethiops COS Cells Diffusion Gold Materials Testing Microscopy, Electron, Scanning Transmission Nanostructures Solutions

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