Voltage-gated potassium channel KCNV2 (Kv8.2) contributes to epilepsy susceptibility.

Jorge BS, Campbell CM, Miller AR, Rutter ED, Gurnett CA, Vanoye CG, George AL, Kearney JA
Proc Natl Acad Sci U S A. 2011 108 (13): 5443-8

PMID: 21402906 · PMCID: PMC3069171 · DOI:10.1073/pnas.1017539108

Mutations in voltage-gated ion channels are responsible for several types of epilepsy. Genetic epilepsies often exhibit variable severity in individuals with the same mutation, which may be due to variation in genetic modifiers. The Scn2a(Q54) transgenic mouse model has a sodium channel mutation and exhibits epilepsy with strain-dependent severity. We previously mapped modifier loci that influence Scn2a(Q54) phenotype severity and identified Kcnv2, encoding the voltage-gated potassium channel subunit Kv8.2, as a candidate modifier. In this study, we demonstrate a threefold increase in hippocampal Kcnv2 expression associated with more severe epilepsy. In vivo exacerbation of the phenotype by Kcnv2 transgenes supports its identification as an epilepsy modifier. The contribution of KCNV2 to human epilepsy susceptibility is supported by identification of two nonsynonymous variants in epilepsy patients that alter function of Kv2.1/Kv8.2 heterotetrameric potassium channels. Our results demonstrate that altered potassium subunit function influences epilepsy susceptibility and implicate Kcnv2 as an epilepsy gene.

MeSH Terms (15)

Amino Acid Sequence Animals Epilepsy Female Hippocampus Humans Male Mice Mice, Inbred C57BL Mutation Patch-Clamp Techniques Phenotype Potassium Channels, Voltage-Gated Protein Isoforms Transgenes

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