A highly immunogenic tumor transfected with a murine transforming growth factor type beta 1 cDNA escapes immune surveillance.

Torre-Amione G, Beauchamp RD, Koeppen H, Park BH, Schreiber H, Moses HL, Rowley DA
Proc Natl Acad Sci U S A. 1990 87 (4): 1486-90

PMID: 2137615 · PMCID: PMC53500 · DOI:10.1073/pnas.87.4.1486

A highly immunogenic C3H-derived UV-induced tumor was cotransfected with a murine transforming growth factor type beta 1 (TGF-beta 1) cDNA and a neomycin-resistance gene. Stable clones were isolated and used in vitro and in vivo to determine the effects of endogenously produced TGF-beta on cytolytic T-lymphocyte (CTL) responses. Tumor cells producing TGF-beta, though retaining expression for class I major histocompatibility complex molecules and the tumor-specific antigen, did not stimulate primary CTL responses in vitro and were not effective in vivo for directly stimulating primary CTL or in priming for CTL responses. Furthermore, TGF-beta-producing tumors grew progressively in transiently immunosuppressed mice without losing the tumor antigen; thus, TGF-beta produced by tumors may promote escape from immune surveillance.

MeSH Terms (21)

Animals Cells, Cultured Cell Transformation, Neoplastic Clone Cells Cytotoxicity, Immunologic Exons Female Fibrosarcoma Immunity, Cellular Immunologic Surveillance Lymphocyte Culture Test, Mixed Mice Mice, Inbred C3H Mice, Nude Neoplasms, Radiation-Induced Plasmids Restriction Mapping Sarcoma, Experimental Transfection Transforming Growth Factors Ultraviolet Rays

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