Linkage analysis of the human dopamine beta-hydroxylase gene.

Perry SE, Summar ML, Phillips JA, Robertson D
Genomics. 1991 10 (2): 493-5

PMID: 2071155 · DOI:10.1016/0888-7543(91)90339-g

The human gene for dopamine beta-hydroxylase (D beta H) has been mapped to chromosome 9q34. Using polymerase chain reaction amplification of exon 11 of the D beta H gene followed by digestion of the reaction products with FnuDII (BstUI), we detected a low-frequency restriction fragment length polymorphism (RFLP). The CEPH panel of family DNAs was genotyped for this RFLP, enabling us to determine the linkage relationships between D beta H and four other loci previously mapped to human chromosome 9q. We obtained two-point recombination frequencies (theta) between D beta H and arginosuccinate synthetase (theta = 0, LOD = 7.37), the ABO blood group locus (theta = 0, LOD = 4.5), CRI-P111 (theta = 0, LOD = 2.1), and D9S31 (theta = .06, LOD = 2.81).

MeSH Terms (11)

Base Sequence Chromosome Mapping Chromosomes, Human, Pair 9 DNA Dopamine beta-Hydroxylase Exons Genetic Linkage Humans Lod Score Molecular Sequence Data Polymerase Chain Reaction

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