1H NMR study of renal trimethylamine responses to dehydration and acute volume loading in man.

Avison MJ, Rothman DL, Nixon TW, Long WS, Siegel NJ
Proc Natl Acad Sci U S A. 1991 88 (14): 6053-7

PMID: 2068084 · PMCID: PMC52020 · DOI:10.1073/pnas.88.14.6053

We have used volume-localized 1H NMR spectroscopy to detect and measure changes in medullary trimethylamines (TMAs) in the human kidney in vivo. Localized water-suppressed 1H spectra were collected from a volume of interest located within the renal medulla by using a stimulated echo-based localization scheme. The principal resonances in the medullary 1H spectrum were residual water (4.7 ppm), lipid (0.9-1.4 ppm), and TMAs (3.25 ppm). The TMA line width was 7-15 Hz before filtering, and the signal-to-noise ratio was 40:1. In four normal volunteers, 15 hr of dehydration led to a significant increase in urine osmolality and decrease in body weight and an increase in medullary TMAs. A subsequent water load [20 ml.(kg of body weight)-1] caused a transient water diuresis, a return to euvolemic body weight, and a significant reduction in medullary TMAs within 4 hr. These results suggest that TMAs may play an osmoregulatory role in the medulla of the normal human kidney.

MeSH Terms (11)

Adult Dehydration Drinking Humans Hydrogen Kidney Kidney Medulla Magnetic Resonance Spectroscopy Male Methylamines Water

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