An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.

Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K
Genesis. 2010 48 (8): 512-20

PMID: 20506501 · DOI:10.1002/dvg.20641

Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57Bl/6 ES cells used in the EUCOMM and KOMP systematic knock-out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient.

(c) 2010 Wiley-Liss, Inc.

MeSH Terms (21)

Animals Base Sequence Binding Sites Cell Line Cells, Cultured DNA Nucleotidyltransferases Embryo, Mammalian Embryonic Stem Cells Female Fibroblasts Gene Expression Regulation, Developmental Gene Knockout Techniques Green Fluorescent Proteins Isoenzymes Male Mice Mice, Inbred C57BL Mice, Transgenic Plasmids Recombination, Genetic Transfection

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