Analysis of protein expression regulated by the Helicobacter pylori ArsRS two-component signal transduction system.

Loh JT, Gupta SS, Friedman DB, Krezel AM, Cover TL
J Bacteriol. 2010 192 (8): 2034-43

PMID: 20154125 · PMCID: PMC2849440 · DOI:10.1128/JB.01703-08

Previous studies have shown that the Helicobacter pylori ArsRS two-component signal transduction system contributes to acid-responsive gene expression. To identify additional members of the ArsRS regulon and further investigate the regulatory role of the ArsRS system, we analyzed protein expression in wild-type and arsS null mutant strains. Numerous proteins were differentially expressed in an arsS mutant strain compared to a wild-type strain when the bacteria were cultured at pH 5.0 and also when they were cultured at pH 7.0. Genes encoding 14 of these proteins were directly regulated by the ArsRS system, based on observed binding of ArsR to the relevant promoter regions. The ArsRS-regulated proteins identified in this study contribute to acid resistance (urease and amidase), acetone metabolism (acetone carboxylase), resistance to oxidative stress (thioredoxin reductase), quorum sensing (Pfs), and several other functions. These results provide further definition of the ArsRS regulon and underscore the importance of the ArsRS system in regulating expression of H. pylori proteins during bacterial growth at both neutral pH and acidic pH.

MeSH Terms (16)

Amidohydrolases Bacterial Proteins Carboxy-Lyases Electrophoresis Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial Helicobacter pylori Hydrogen-Ion Concentration Mass Spectrometry Promoter Regions, Genetic Protein Binding Proteomics Signal Transduction Thioredoxin-Disulfide Reductase Trans-Activators Urease

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