Molecular analysis of tumor margins by MALDI mass spectrometry in renal carcinoma.

Oppenheimer SR, Mi D, Sanders ME, Caprioli RM
J Proteome Res. 2010 9 (5): 2182-90

PMID: 20141219 · PMCID: PMC2866033 · DOI:10.1021/pr900936z

The rate of tumor recurrence post resection suggests that there are underlying molecular changes in nearby histologically normal tissue that go undetected by conventional diagnostic methods that utilize contrast agents and immunohistochemistry. MALDI MS is a molecular technology that has the specificity and sensitivity to monitor and identify molecular species indicative of these changes. The current study utilizes this technology to assess molecular distributions within a tumor and adjacent normal tissue in clear cell renal cell carcinoma biopsies. Results indicate that the histologically normal tissue adjacent to the tumor expresses many of the molecular characteristics of the tumor. Proteins of the mitochondrial electron transport system are examples of such distributions. This work demonstrates the utility of MALDI MS for the analysis of tumor tissue in the elucidation of aberrant molecular changes in the tumor microenvironment.

MeSH Terms (16)

Algorithms Artificial Intelligence Carcinoma, Renal Cell Diagnostic Imaging Electron Transport Complex IV Histocytochemistry Humans Image Interpretation, Computer-Assisted Kidney Neoplasms Molecular Diagnostic Techniques Neoplasm Invasiveness Protein Array Analysis Proteome Proteomics Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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