Assessing the application of tissue microarray technology to kidney research.

Zhang MZ, Su Y, Yao B, Zheng W, Decaestecker M, Harris RC
J Histochem Cytochem. 2010 58 (5): 413-20

PMID: 20086233 · PMCID: PMC2857813 · DOI:10.1369/jhc.2009.954966

Tissue microarray (TMA) is a new high-throughput method that enables simultaneous analysis of the profiles of protein expression in multiple tissue samples. TMA technology has not previously been adapted for physiological and pathophysiological studies of rodent kidneys. We have evaluated the validity and reliability of using TMA to assess protein expression in mouse and rat kidneys. A representative TMA block that we have produced included: (1) mouse and rat kidney cortex, outer medulla, and inner medulla fixed with different fixatives; (2) rat kidneys at different stages of development fixed with different fixatives; (3) mouse and rat kidneys with different physiological or pathophysiological treatments; and (4) built-in controls. As examples of the utility, immunostaining for cyclooxygenase-2, renin, Tamm Horsfall protein, aquaporin-2, connective tissue growth factor, and synaptopodin was carried out with kidney TMA slides. Quantitative analysis of cyclooxygense-2 expression in kidneys confirms that individual cores provide meaningful representations comparable to whole-kidney sections. These studies show that kidney TMA technique is a promising and useful tool for investigating the expression profiles of proteins of interest in rodent kidneys under different physiological and pathophysiological conditions.

MeSH Terms (15)

Angiotensin II Type 1 Receptor Blockers Animals Aquaporin 2 Connective Tissue Growth Factor Cyclooxygenase 2 Fixatives Immunohistochemistry Kidney Mice Mucoproteins Rats Renin Tissue Array Analysis Tissue Fixation Uromodulin

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