Repressive BMP2 gene regulatory elements near the BMP2 promoter.

Jiang S, Chandler RL, Fritz DT, Mortlock DP, Rogers MB
Biochem Biophys Res Commun. 2010 392 (2): 124-8

PMID: 20060812 · PMCID: PMC2822113 · DOI:10.1016/j.bbrc.2009.12.157

The level of bone morphogenetic protein 2 (BMP2) profoundly influences essential cell behaviors such as proliferation, differentiation, apoptosis, and migration. The spatial and temporal pattern of BMP2 synthesis, particular in diverse embryonic cells, is highly varied and dynamic. We have identified GC-rich sequences within the BMP2 promoter region that strongly repress gene expression. These elements block the activity of a highly conserved, osteoblast enhancer in response to FGF2 treatment. Both positive and negative gene regulatory elements control BMP2 synthesis. Detecting and mapping the repressive motifs is essential because they impede the identification of developmentally regulated enhancers necessary for normal BMP2 patterns and concentration.

2010 Elsevier Inc. All rights reserved.

MeSH Terms (18)

Animals Base Sequence Bone Morphogenetic Protein 2 Cell Line Conserved Sequence Enhancer Elements, Genetic Fibroblast Growth Factor 2 GC Rich Sequence Gene Expression Regulation, Developmental Genes, Reporter HeLa Cells Humans Mice Molecular Sequence Data Osteoblasts Promoter Regions, Genetic Regulatory Elements, Transcriptional Repressor Proteins

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