Functional delivery of a membrane protein into oocyte membranes using bicelles.

Kang C, Vanoye CG, Welch RC, Van Horn WD, Sanders CR
Biochemistry. 2010 49 (4): 653-5

PMID: 20044833 · PMCID: PMC2811756 · DOI:10.1021/bi902155t

Voltage-gated potassium channel modulatory membrane protein KCNE3 was overexpressed and purified into both micelles and bicelles. Remarkably, microinjection of KCNE3 in bicelles into Xenopus oocytes resulted in functional co-assembly with the human KCNQ1 channel expressed therein. Microinjection of LMPC micelles containing KCNE3 did not result in channel modulation, indicating that bicelles sometimes succeed at delivering a membrane protein into a cellular membrane when classical micelles fail. Backbone NMR resonance assignments were completed for KCNE3 in both bicelles and LMPC, indicating that the secondary structure distribution in KCNE3's N-terminus and transmembrane domains exhibits only modest differences from that of KCNE1, even though these KCNE family members have very different effects on KCNQ1 channel function.

MeSH Terms (13)

Amino Acid Sequence Animals Humans KCNQ1 Potassium Channel Membrane Potentials Membrane Proteins Micelles Microinjections Models, Biological Molecular Sequence Data Oocytes Potassium Channels, Voltage-Gated Xenopus

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