High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.

Demian DJ, Clugston SL, Foster MM, Rameh L, Sarkes D, Townson SA, Yang L, Zhang M, Charlton ME
J Biomol Screen. 2009 14 (7): 838-44

PMID: 19641220 · DOI:10.1177/1087057109339205

MeSH Terms (17)

Adenosine Triphosphate Calorimetry Cell-Free System Chromatography, Thin Layer Crystallography, X-Ray Densitometry High-Throughput Screening Assays Humans Inhibitory Concentration 50 Kinetics Lipid Metabolism Liposomes Phosphorus Radioisotopes Phosphotransferases Protein Kinase Inhibitors Reproducibility of Results Substrate Specificity

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