Electrochemical sensor for multiplex biomarkers detection.

Wei F, Patel P, Liao W, Chaudhry K, Zhang L, Arellano-Garcia M, Hu S, Elashoff D, Zhou H, Shukla S, Shah F, Ho CM, Wong DT
Clin Cancer Res. 2009 15 (13): 4446-52

PMID: 19509137 · PMCID: PMC2799532 · DOI:10.1158/1078-0432.CCR-09-0050

PURPOSE - Multiplexing assay of biomarkers at the point-of-care is an elusive goal for molecular diagnostics.

EXPERIMENTAL DESIGN - Here, we report an electrochemical (EC) sensor for oral cancer detection based on the simultaneous detection of two salivary biomarkers: interleukin (IL)-8 mRNA and IL-8 protein.

RESULTS - Under the multiplexing mode, the limit of detection of salivary IL-8 mRNA reaches to 3.9 fM and 7.4 pg/mL for IL-8 protein in saliva. Multiplex assay of these 2 biomarkers directly from 28 cancer and 28 matched control saliva samples shows significant difference between the two groups. From the receiver operating characteristic analysis, the EC sensor yields around 90% sensitivity and specificity for both IL-8 mRNA and IL-8 protein, which are very close to the data measured by traditional assays (ELISA and PCR) with the same group of saliva. Combined IL-8 mRNA and protein show better AUC compared with single biomarker.

CONCLUSIONS - We show, for the first time, concurrently multiplexing detection of salivary mRNA and protein biomarkers using point-of-care EC sensor.

MeSH Terms (15)

Biomarkers, Tumor Biosensing Techniques Case-Control Studies Early Detection of Cancer Electrochemical Techniques Enzyme-Linked Immunosorbent Assay Humans Interleukin-18 Models, Biological Mouth Neoplasms Polymerase Chain Reaction RNA, Messenger ROC Curve Saliva Sensitivity and Specificity

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