Delineation of a carcinogenic Helicobacter pylori proteome.

Franco AT, Friedman DB, Nagy TA, Romero-Gallo J, Krishna U, Kendall A, Israel DA, Tegtmeyer N, Washington MK, Peek RM
Mol Cell Proteomics. 2009 8 (8): 1947-58

PMID: 19470446 · PMCID: PMC2722763 · DOI:10.1074/mcp.M900139-MCP200

Helicobacter pylori is the strongest known risk factor for gastric adenocarcinoma, yet only a fraction of infected persons ever develop cancer. The extensive genetic diversity inherent to this pathogen has precluded comprehensive analyses of constituents that mediate carcinogenesis. We previously reported that in vivo adaptation of a non-carcinogenic H. pylori strain endowed the output derivative with the ability to induce adenocarcinoma, providing a unique opportunity to identify proteins selectively expressed by an oncogenic H. pylori strain. Using a global proteomics DIGE/MS approach, a novel missense mutation of the flagellar protein FlaA was identified that affects structure and function of this virulence-related organelle. Among 25 additional differentially abundant proteins, this approach also identified new proteins previously unassociated with gastric cancer, generating a profile of H. pylori proteins to use in vaccine development and for screening persons infected with strains most likely to induce severe disease.

MeSH Terms (17)

Animals Bacterial Proteins Blotting, Western Cell Line Electrophoresis, Gel, Two-Dimensional Flagellin Gerbillinae Helicobacter Infections Helicobacter pylori Humans Male Microscopy, Electron, Transmission Mutation, Missense Proteome Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stomach Neoplasms

Connections (4)

This publication is referenced by other Labnodes entities:

Links