Photoconversion in orange and red fluorescent proteins.

Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
Nat Methods. 2009 6 (5): 355-8

PMID: 19363494 · PMCID: PMC2675661 · DOI:10.1038/nmeth.1319

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

MeSH Terms (9)

Cell Survival HeLa Cells Humans Hydrogen-Ion Concentration Luminescent Proteins Microscopy, Fluorescence Photochemical Processes Spectrometry, Fluorescence Transfection

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