Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis.

Anderson MA, Jodoin JN, Lee E, Hales KG, Hays TS, Lee LA
Mol Biol Cell. 2009 20 (11): 2709-21

PMID: 19357193 · PMCID: PMC2688550 · DOI:10.1091/mbc.e08-12-1165

Spermatogenesis uses mitotic and meiotic cell cycles coordinated with growth and differentiation programs to generate functional sperm. Our analysis of a Drosophila mutant has revealed that asunder (asun), which encodes a conserved protein, is an essential regulator of spermatogenesis. asun spermatocytes arrest during prophase of meiosis I. Strikingly, arrested spermatocytes contain free centrosomes that fail to stably associate with the nucleus. Spermatocytes that overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cytokinesis. Furthermore, the centriole-derived basal body is detached from the nucleus in asun postmeiotic spermatids, resulting in abnormalities later in spermatogenesis. We find that asun spermatocytes and spermatids exhibit drastic reduction of perinuclear dynein-dynactin, a microtubule motor complex. We propose a model in which asun coordinates spermatogenesis by promoting dynein-dynactin recruitment to the nuclear surface, a poorly understood process required for nucleus-centrosome coupling at M phase entry and fidelity of meiotic divisions.

MeSH Terms (26)

Animals Animals, Genetically Modified Cell Cycle Proteins Cell Nucleus Chromosome Segregation Drosophila melanogaster Drosophila Proteins Dynactin Complex Dyneins Fertility Green Fluorescent Proteins HeLa Cells Humans Immunoblotting Infertility, Male Male Meiosis Microscopy, Fluorescence Microtubule-Associated Proteins Mutation Reverse Transcriptase Polymerase Chain Reaction Spermatids Spermatocytes Spermatogenesis Spindle Apparatus Transfection

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