Single molecule-sensitive probes for imaging RNA in live cells.

Santangelo PJ, Lifland AW, Curt P, Sasaki Y, Bassell GJ, Lindquist ME, Crowe JE
Nat Methods. 2009 6 (5): 347-9

PMID: 19349979 · PMCID: PMC4297622 · DOI:10.1038/nmeth.1316

To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.

MeSH Terms (29)

Actin-Related Protein 2 Actins Animals Avian Proteins Bacterial Proteins Biotin Carbocyanines Cell Line, Tumor Cell Membrane Permeability Cells, Cultured Cell Survival Chick Embryo DNA-Binding Proteins Fibroblasts Fluorescent Dyes Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Molecular Probe Techniques Oligonucleotide Probes Poly(A)-Binding Proteins Respiratory Syncytial Virus, Human RNA RNA, Messenger RNA-Binding Proteins Streptavidin Streptolysins T-Cell Intracellular Antigen-1

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