Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish.

Kim JD, Chun HS, Kim SH, Kim HS, Kim YS, Kim MJ, Shin J, Rhee M, Yeo SY, Huh TL
Biochem Biophys Res Commun. 2009 381 (4): 717-21

PMID: 19258008 · DOI:10.1016/j.bbrc.2009.02.135

During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.

MeSH Terms (11)

Animals Animals, Genetically Modified Frizzled Receptors Homeodomain Proteins Prosencephalon Receptors, G-Protein-Coupled Somites Transcription Factors Wnt Proteins Zebrafish Zebrafish Proteins

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