Lipopolysaccharide-dependent interaction between PU.1 and c-Jun determines production of lipocalin-type prostaglandin D synthase and prostaglandin D2 in macrophages.

Joo M, Kwon M, Cho YJ, Hu N, Pedchenko TV, Sadikot RT, Blackwell TS, Christman JW
Am J Physiol Lung Cell Mol Physiol. 2009 296 (5): L771-9

PMID: 19181746 · PMCID: PMC2681347 · DOI:10.1152/ajplung.90320.2008

Previously, we reported that expression of lipocalin-prostaglandin D synthase (L-PGDS) is inducible in macrophages and protects from Pseudomonas pneumonia. Here, we investigated the mechanism by which L-PGDS gene expression is induced in macrophages. A promoter analysis of the murine L-PGDS promoter located a binding site of PU.1, a transcription factor essential for macrophage development and inflammatory gene expression. A chromatin immunoprecipitation assay showed that PU.1 bound to the cognate site in the endogenous L-PGDS promoter in response to LPS. Overexpression of PU.1, but not of PU.1(S148A), a mutant inert to casein kinase II (CKII) or NF-kappaB-inducing kinase (NIK), induced L-PGDS in RAW 264.7 cells. Conversely, siRNA silencing of PU.1 expression blunted productions of L-PGDS and prostaglandin D2 (PGD(2)). LPS treatment induced formation of the complex of PU.1 and cJun on the PU.1 site, but inactivation of cJun by treatment with JNK or p38 kinase inhibitor abolished the complex, and suppressed PU.1 transcriptional activity for L-PGDS gene expression. Together, these results show that PU.1, activated by CKII or NIK, cooperates with MAPK-activated cJun to maximally induce L-PGDS expression in macrophages following LPS treatment, and suggest that PU.1 participates in innate immunity through the production of L-PGDS and PGD(2).

MeSH Terms (24)

Animals Base Sequence Binding Sites Cell Line Female Humans Intramolecular Oxidoreductases JNK Mitogen-Activated Protein Kinases Lipocalins Lipopolysaccharides Macrophages Male Mice Mice, Inbred C57BL Models, Biological Molecular Sequence Data p38 Mitogen-Activated Protein Kinases Promoter Regions, Genetic Prostaglandin D2 Protein Binding Proto-Oncogene Proteins Proto-Oncogene Proteins c-jun Trans-Activators Transcriptional Activation

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