Free cholesterol accumulation in macrophage membranes activates Toll-like receptors and p38 mitogen-activated protein kinase and induces cathepsin K.

Sun Y, Ishibashi M, Seimon T, Lee M, Sharma SM, Fitzgerald KA, Samokhin AO, Wang Y, Sayers S, Aikawa M, Jerome WG, Ostrowski MC, Bromme D, Libby P, Tabas IA, Welch CL, Tall AR
Circ Res. 2009 104 (4): 455-65

PMID: 19122179 · PMCID: PMC2680702 · DOI:10.1161/CIRCRESAHA.108.182568

The molecular events linking lipid accumulation in atherosclerotic plaques to complications such as aneurysm formation and plaque disruption are poorly understood. BALB/c-Apoe(-/-) mice bearing a null mutation in the Npc1 gene display prominent medial erosion and atherothrombosis, whereas their macrophages accumulate free cholesterol in late endosomes and show increased cathepsin K (Ctsk) expression. We now show increased cathepsin K immunostaining and increased cysteinyl proteinase activity using near infrared fluorescence imaging over proximal aortas of Apoe(-/-), Npc1(-/-) mice. In mechanistic studies, cholesterol loading of macrophage plasma membranes (cyclodextrin-cholesterol) or endosomal system (AcLDL+U18666A or Npc1 null mutation) activated Toll-like receptor (TLR) signaling, leading to sustained phosphorylation of p38 mitogen-activated protein kinase and induction of p38 targets, including Ctsk, S100a8, Mmp8, and Mmp14. Studies in macrophages from knockout mice showed major roles for TLR4, following plasma membrane cholesterol loading, and for TLR3, after late endosomal loading. TLR signaling via p38 led to phosphorylation and activation of the transcription factor Microphthalmia transcription factor, acting at E-box elements in the Ctsk promoter. These studies suggest that free cholesterol enrichment of either plasma or endosomal membranes in macrophages leads to activation of signaling via various TLRs, prolonged p38 mitogen-activated protein kinase activation, and induction of Mmps, Ctsk, and S100a8, potentially contributing to plaque complications.

MeSH Terms (33)

Adaptor Proteins, Vesicular Transport Animals Aorta Apolipoproteins E Calgranulin A Cathepsin K Cathepsins Cell Membrane Cells, Cultured Cholesterol E-Box Elements Endosomes Enzyme Induction Humans Macrophages Matrix Metalloproteinase 8 Matrix Metalloproteinase 14 Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Knockout Microphthalmia-Associated Transcription Factor p38 Mitogen-Activated Protein Kinases Phosphorylation Promoter Regions, Genetic Proteins rab GTP-Binding Proteins Receptor Activator of Nuclear Factor-kappa B S100 Proteins Signal Transduction Time Factors Toll-Like Receptor 3 Toll-Like Receptor 4

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