Transcriptional dynamics of endodermal organ formation.

Sherwood RI, Chen TY, Melton DA
Dev Dyn. 2009 238 (1): 29-42

PMID: 19097184 · PMCID: PMC3756511 · DOI:10.1002/dvdy.21810

Although endodermal organs including the liver, pancreas, and intestine are of significant therapeutic interest, the mechanism by which the endoderm is divided into organ domains during embryogenesis is not well understood. To better understand this process, global gene expression profiling was performed on early endodermal organ domains. This global analysis was followed up by dynamic immunofluorescence analysis of key transcription factors, uncovering novel expression patterns as well as cell surface proteins that allow prospective isolation of specific endodermal organ domains. Additionally, a repressive interaction between Cdx2 and Sox2 was found to occur at the prospective stomach-intestine border, with the hepatic and pancreatic domains forming at this boundary, and Hlxb9 was revealed to have graded expression along the dorsal-ventral axis. These results contribute to understanding the mechanism of endodermal organogenesis and should assist efforts to replicate this process using pluripotent stem cells.

Copyright (c) 2008 Wiley-Liss, Inc.

MeSH Terms (12)

Animals Body Patterning Embryo, Mammalian Endoderm Gene Expression Profiling Gene Expression Regulation, Developmental Homeodomain Proteins Mice Morphogenesis Oligonucleotide Array Sequence Analysis Transcription, Genetic Transcription Factors

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