Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.

Nyman LR, Wells KS, Head WS, McCaughey M, Ford E, Brissova M, Piston DW, Powers AC
J Clin Invest. 2008 118 (11): 3790-7

PMID: 18846254 · PMCID: PMC2564611 · DOI:10.1172/JCI36209

The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods.

MeSH Terms (9)

Animals Diagnostic Imaging Hemodynamics Imaging, Three-Dimensional Islets of Langerhans Mice Mice, Transgenic Microscopy, Confocal Time Factors

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