Nucleosome organization in the Drosophila genome.

Mavrich TN, Jiang C, Ioshikhes IP, Li X, Venters BJ, Zanton SJ, Tomsho LP, Qi J, Glaser RL, Schuster SC, Gilmour DS, Albert I, Pugh BF
Nature. 2008 453 (7193): 358-62

PMID: 18408708 · PMCID: PMC2735122 · DOI:10.1038/nature06929

Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.

MeSH Terms (13)

Animals Conserved Sequence Drosophila melanogaster Gene Expression Regulation Genes, Insect Genome, Insect Histones Nucleosomes Promoter Regions, Genetic RNA Polymerase II Saccharomyces cerevisiae Transcription, Genetic Transcription Initiation Site

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