Sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence.

Zelent B, Odili S, Buettger C, Shiota C, Grimsby J, Taub R, Magnuson MA, Vanderkooi JM, Matschinsky FM
Biochem J. 2008 413 (2): 269-80

PMID: 18370929 · DOI:10.1042/BJ20071718

MeSH Terms (15)

Binding Sites Carbohydrates Escherichia coli Glucokinase Glutathione Transferase Humans Kinetics Molecular Conformation Mutation Protein Binding Protein Conformation Recombinant Fusion Proteins Recombinant Proteins Spectrometry, Fluorescence Tryptophan

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