High-resolution high-speed panoramic cardiac imaging system.

Evertson DW, Holcomb MR, Eames MC, Bray MA, Sidorov VY, Xu J, Wingard H, Dobrovolny HM, Woods MC, Gauthier DJ, Wikswo JP
IEEE Trans Biomed Eng. 2008 55 (3): 1241-3

PMID: 18334422 · PMCID: PMC2561274 · DOI:10.1109/TBME.2007.912417

A panoramic cardiac imaging system consisting of three high-speed CCD cameras has been developed to image the surface electrophysiology of a rabbit heart via fluorescence imaging using a voltage-sensitive fluorescent dye. A robust, unique mechanical system was designed to accommodate the three cameras and to adapt to the requirements of future experiments. A unified computer interface was created for this application - a single workstation controls all three CCD cameras, illumination, stimulation, and a stepping motor that rotates the heart. The geometric reconstruction algorithms were adapted from a previous cardiac imaging system. We demonstrate the system by imaging a polymorphic cardiac tachycardia.

MeSH Terms (11)

Algorithms Animals Arrhythmias, Cardiac Body Surface Potential Mapping Equipment Design Equipment Failure Analysis Heart Conduction System Image Enhancement Imaging, Three-Dimensional Microscopy, Fluorescence Rabbits

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