The inhibition of tumor cell intravasation and subsequent metastasis via regulation of in vivo tumor cell motility by the tetraspanin CD151.

Zijlstra A, Lewis J, Degryse B, Stuhlmann H, Quigley JP
Cancer Cell. 2008 13 (3): 221-34

PMID: 18328426 · PMCID: PMC3068919 · DOI:10.1016/j.ccr.2008.01.031

In vivo tumor cell migration through integrin-dependent pathways is key to the metastatic behavior of malignant cells. Using quantitative in vivo assays and intravital imaging, we assessed the impact of cell migration, regulated by the integrin-associated tetraspanin CD151, on spontaneous human tumor cell metastasis. We demonstrate that promoting immobility through a CD151-specific metastasis blocking mAb prevents tumor cell dissemination by inhibiting intravasation without affecting primary tumor growth, tumor cell arrest, extravasation, or growth at the secondary site. In vivo, this loss of migration is the result of enhanced tumor cell-matrix interactions, promoted by CD151, which prevent dissociation by individual cells and leads to a subsequent inhibition of invasion and intravasation at the site of the primary tumor.

MeSH Terms (27)

Animals Antibodies, Monoclonal Antigens, CD Binding Sites, Antibody Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Chick Embryo Chorioallantoic Membrane Extracellular Matrix Humans Mice Mice, Knockout Mice, SCID Microscopy, Fluorescence Microscopy, Video Neoplasm Invasiveness Neoplasm Metastasis Neoplasms, Experimental Polymerase Chain Reaction Recombinant Fusion Proteins RNA, Small Interfering RNA Interference Tetraspanin 24 Time Factors Transfection

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