Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A.

Mudryj M, Devoto SH, Hiebert SW, Hunter T, Pines J, Nevins JR
Cell. 1991 65 (7): 1243-53

PMID: 1829647 · DOI:10.1016/0092-8674(91)90019-u

We have examined E2F binding activity in extracts of synchronized NIH 3T3 cells. During the G0 to G1 transition, there is a marked increase in the level of active E2F. Subsequently, there are changes in the nature of E2F-containing complexes. A G1-specific complex increases in abundance, disappears, and is then replaced by another complex as S phase begins. Analysis of extracts of thymidine-blocked cells confirms that the complexes are cell cycle regulated. We also show that the cyclin A protein is a component of the S phase complex. Each complex can be dissociated by the adenovirus E1A 12S product, releasing free E2F. The release of E2F from the cyclin A complex coincides with the stimulation of an E2F-dependent promoter. We suggest that these interactions control the activity of E2F and that disruption of the complexes by E1A contributes to a loss of cellular proliferation control.

MeSH Terms (17)

Adenovirus Early Proteins Animals Carrier Proteins Cell Cycle Cell Cycle Proteins Cell Line Cyclins DNA-Binding Proteins E2F Transcription Factors Macromolecular Substances Mice Oncogene Proteins, Viral Protein Binding Retinoblastoma-Binding Protein 1 Time Factors Transcription Factor DP1 Transcription Factors

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