Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation.

de Curtis I, Quaranta V, Tamura RN, Reichardt LF
J Cell Biol. 1991 113 (2): 405-16

PMID: 1826298 · PMCID: PMC2288948 · DOI:10.1083/jcb.113.2.405

The integrin alpha 6 beta 1 is a prominent laminin receptor used by many cell types. In the present work, we isolate clones and determine the primary sequence of the chick integrin alpha 6 subunit. We show that alpha 6 beta 1 is a prominent integrin expressed by cells in the developing chick retina. Between embryonic days 6 and 12, both retinal ganglion cells and other retinal neurons lose selected integrin functions, including the ability to attach and extend neurites on laminin. In retinal ganglion cells, we show that this is correlated with a dramatic decrease in alpha 6 mRNA and protein, suggesting that changes in gene expression account for the developmental regulation of the interactions of these neurons with laminin. In other retinal neurons the expression of alpha 6 mRNA and protein remains high while function is lost, suggesting that the function of the alpha 6 beta 1 heterodimer in these cells is regulated by posttranslational mechanisms.

MeSH Terms (19)

Amino Acid Sequence Animals Base Sequence Chick Embryo Cloning, Molecular DNA Gene Expression Regulation Humans Integrins Molecular Sequence Data Precipitin Tests Protein Biosynthesis Receptors, Immunologic Receptors, Laminin Restriction Mapping Retina Retinal Ganglion Cells Sequence Homology, Nucleic Acid Transcription, Genetic

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