Maize centromere mapping: a comparison of physical and genetic strategies.

Okagaki RJ, Jacobs MS, Stec AO, Kynast RG, Buescher E, Rines HW, Vales MI, Riera-Lizarazu O, Schneerman M, Doyle G, Friedman KL, Staub RW, Weber DF, Kamps TL, Amarillo IF, Chase CD, Bass HW, Phillips RL
J Hered. 2008 99 (2): 85-93

PMID: 18216028 · DOI:10.1093/jhered/esm111

Centromere positions on 7 maize chromosomes were compared on the basis of data from 4 to 6 mapping techniques per chromosome. Centromere positions were first located relative to molecular markers by means of radiation hybrid lines and centric fission lines recovered from oat-maize chromosome addition lines. These centromere positions were then compared with new data from centric fission lines recovered from maize plants, half-tetrad mapping, and fluorescence in situ hybridizations and to data from earlier studies. Surprisingly, the choice of mapping technique was not the critical determining factor. Instead, on 4 chromosomes, results from all techniques were consistent with a single centromere position. On chromosomes 1, 3, and 6, centromere positions were not consistent even in studies using the same technique. The conflicting centromere map positions on chromosomes 1, 3, and 6 could be explained by pericentric inversions or alternative centromere positions on these chromosomes.

MeSH Terms (5)

Centromere Chromosome Mapping Chromosomes, Plant In Situ Hybridization, Fluorescence Zea mays

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