Overexpression of EPHA2 receptor destabilizes adherens junctions via a RhoA-dependent mechanism.

Fang WB, Ireton RC, Zhuang G, Takahashi T, Reynolds A, Chen J
J Cell Sci. 2008 121 (Pt 3): 358-68

PMID: 18198190 · DOI:10.1242/jcs.017145

EPHA2 receptor tyrosine kinase is overexpressed in several human cancer types and promotes malignancy. However, the mechanisms by which EPHA2 promotes tumor progression are not completely understood. Here we report that overexpression of a wild-type EPHA2, but not a signaling-defective cytoplasmic truncation mutant (DeltaC), in human mammary epithelial cells weakens E-cadherin-mediated cell-cell adhesion. Interestingly, the total level of cadherins and the composition of the adherens junction complexes were not affected, nor was the tyrosine phosphorylation of the cadherin complex components changed. By contrast, RhoA GTPase activity was significantly affected by modulating the EPHA2 activity in MCF-10A cells. Treatment with a ROCK kinase inhibitor rescued cell-cell adhesion defects in EPHA2-overexpressing cells, whereas expression of constitutively activated Rho disrupted adherens junctions in DeltaC-expressing cells. EPHA2-dependent Rho activation and destabilization of adherens junctions appeared to be regulated via a signaling pathway involving Src kinase, low molecular weight phosphotyrosine phosphatase (LMW-PTP) and p190 RhoGAP. EPHA2 interacted with both Src and LMW-PTP, and the interactions increased in EPHA2-overexpressing cells. In addition, LMW-PTP phosphatase activity was elevated, and this elevation was accompanied by a decrease in tyrosine phosphorylation of p190 RhoGAP and destabilization of cell-cell adhesion. Expression of either a dominant negative LMW-PTP mutant, C12S, or a wild-type p190 RhoGAP rescued adhesion defects in EPHA2-overexpressing cells. Together, these data suggest that EPHA2 promotes tumor malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions.

MeSH Terms (20)

Adherens Junctions Cadherins Catenins Cell Adhesion Cell Line Female Gene Expression Guanine Nucleotide Exchange Factors Humans Models, Biological Mutation Phosphorylation Protein Tyrosine Phosphatases Receptor, EphA2 Recombinant Proteins Repressor Proteins rhoA GTP-Binding Protein Sequence Deletion Signal Transduction src-Family Kinases

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