Physiological glucose is critical for optimized neuronal viability and AMPK responsiveness in vitro.

Kleman AM, Yuan JY, Aja S, Ronnett GV, Landree LE
J Neurosci Methods. 2008 167 (2): 292-301

PMID: 17936912 · PMCID: PMC2257477 · DOI:10.1016/j.jneumeth.2007.08.028

Understanding the mechanisms that govern neuronal responses to oxidative and metabolic stress is essential for therapeutic intervention. In vitro modeling is an important approach for these studies, as the metabolic environment influences neuronal responses. Surprisingly, most neuronal culture methods employ conditions that are non-physiological, especially with regards to glucose concentrations, which often exceed 20mM. This concentration is a significant departure from physiological glucose levels, and even several-fold greater than that seen during severe hyperglycemia. The goal of this study was to establish a physiological neuronal culture system that will facilitate the study of neuronal energy metabolism and responses to metabolic stress. We demonstrate that the metabolic environment during preparation, plating, and maintenance of cultures affects neuronal viability and the response of neuronal pathways to changes in energy balance.

MeSH Terms (20)

4-Butyrolactone Adenosine Triphosphate Aminoimidazole Carboxamide AMP-Activated Protein Kinases Analysis of Variance Animals Cells, Cultured Cell Survival Cerebral Cortex Dose-Response Relationship, Drug Embryo, Mammalian Glucose Hypoglycemic Agents Multienzyme Complexes Neurons Protein-Serine-Threonine Kinases Rats Rats, Sprague-Dawley Ribonucleotides Time Factors

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