Characterization of the human skeletal muscle proteome by one-dimensional gel electrophoresis and HPLC-ESI-MS/MS.

Højlund K, Yi Z, Hwang H, Bowen B, Lefort N, Flynn CR, Langlais P, Weintraub ST, Mandarino LJ
Mol Cell Proteomics. 2008 7 (2): 257-67

PMID: 17911086 · PMCID: PMC3836591 · DOI:10.1074/mcp.M700304-MCP200

Changes in protein abundance in skeletal muscle are central to a large number of metabolic and other disorders, including, and perhaps most commonly, insulin resistance. Proteomics analysis of human muscle is an important approach for gaining insight into the biochemical basis for normal and pathophysiological conditions. However, to date, the number of proteins identified by this approach has been limited, with 107 different proteins being the maximum reported so far. Using a combination of one-dimensional gel electrophoresis and high performance liquid chromatography electrospray ionization tandem mass spectrometry, we identified 954 different proteins in human vastus lateralis muscle obtained from three healthy, nonobese subjects. In addition to a large number of isoforms of contractile proteins, we detected all proteins involved in the major pathways of glucose and lipid metabolism in skeletal muscle. Mitochondrial proteins accounted for 22% of all proteins identified, including 55 subunits of the respiratory complexes I-V. Moreover, a number of enzymes involved in endocrine and metabolic signaling pathways as well as calcium homeostasis were identified. These results provide the most comprehensive characterization of the human skeletal muscle proteome to date. These data hold promise for future global assessment of quantitative changes in the muscle proteome of patients affected by disorders involving skeletal muscle.

MeSH Terms (23)

Adult Calcium Chromatography, High Pressure Liquid Contractile Proteins Electron Transport Electrophoresis, Polyacrylamide Gel Extracellular Matrix Proteins Glucose Homeostasis Humans Insulin Insulin-Like Growth Factor I Lipid Metabolism Middle Aged Molecular Weight Muscle, Skeletal Muscle Proteins Oxidative Phosphorylation Protein Transport Proteome Proteomics Spectrometry, Mass, Electrospray Ionization Subcellular Fractions

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