Phosphoinositide kinase-3 status associated with presence or absence of human papillomavirus in head and neck squamous cell carcinomas.

Yarbrough WG, Whigham A, Brown B, Roach M, Slebos R
Int J Radiat Oncol Biol Phys. 2007 69 (2 Suppl): S98-101

PMID: 17848307 · DOI:10.1016/j.ijrobp.2007.04.082

PURPOSE - To investigate phosphoinositide kinase-3 (PI3K) activation in relation to human papillomavirus (HPV) status in head and neck squamous cell carcinoma (HNSCC).

METHODS AND MATERIALS - Gene expression microarray data were analyzed to determine differentially expressed genes between HPV(+) and HPV(-) HNSCC. PIK3CA gene expression was confirmed by quantitative reverse transcriptase-polymerase chain reaction in seven HPV(+) and seven HPV(-) primary HNSCCs. PIK3CA mutation status in three HPV(+) and nine HPV(-) cell lines was determined by polymerase chain reaction amplification of hot spot exons (1, 9, 20) followed by direct sequencing.

RESULTS - PIK3CA was overexpressed in HPV(+)-associated HNSCC compared with the expression in HPV(-) HNSCC. Activation of PIK3CA by mutation was found in 1 of the 12 tested HNSCC cell lines.

CONCLUSION - Activation of PI3K by mutation of PIK3CA is rare in HNSCC cell lines and was not found in three HPV(+) cell lines. One mechanism by which HPV-associated HNSCC might activate PI3K is increased expression of PIK3CA.

MeSH Terms (9)

Carcinoma, Squamous Cell Cell Line, Tumor Enzyme Activation Head and Neck Neoplasms Humans Mutation Neoplasm Proteins Papillomaviridae Phosphatidylinositol 3-Kinases

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