A simple rapid immunoassay for S-adenosylhomocysteine in plasma.

Capdevila A, Burk RF, Freedman J, Frantzen F, Alfheim I, Wagner C
J Nutr Biochem. 2007 18 (12): 827-31

PMID: 17509856 · PMCID: PMC2196216 · DOI:10.1016/j.jnutbio.2007.01.003

The measurement of plasma S-adenosylhomocysteine is a more sensitive indicator of the risk for vascular disease than is plasma homocysteine. Because the level of S-adenosylhomocysteine is normally in the nanomolar range, it has been difficult to measure and necessitated the development of complex fluorometric and mass-spectrophotometric methods. We have now adapted an existing immunoassay used for the measurement of homocysteine to the measurement of S-adenosylhomocysteine in plasma. This assay is sensitive down to the level of less than 0.1 pmol, and there is no interference by S-adenosylmethionine. The assay is carried out in microplates, allows the measurement of 12 samples per plate and can easily be carried out in a 4-h period. The method is applicable to plasma samples having S-adenosylhomocysteine concentrations ranging from 10 to 150 nM without dilution. The mean value for 16 normal subjects by this method was 18.9+/-1.4 nM (S.E.M.), compared with 17.8+/-1.4 nM obtained by a previously described method using two high-performance liquid chromatography columns with fluorescence derivatization. Mean values for seven cirrhotic patients were 46.5+/-3.3 nM by this new method compared with 44.6+/-5.3 by the former method. The ease and speed of this method should allow the widespread measurement of this important metabolite in laboratories without access to sophisticated equipment.

MeSH Terms (7)

Chromatography, High Pressure Liquid Humans Radioimmunoassay Reference Values Reproducibility of Results S-Adenosylhomocysteine Sensitivity and Specificity

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