A major zebrafish polymorphism resource for genetic mapping.

Bradley KM, Elmore JB, Breyer JP, Yaspan BL, Jessen JR, Knapik EW, Smith JR
Genome Biol. 2007 8 (4): R55

PMID: 17428331 · PMCID: PMC1896001 · DOI:10.1186/gb-2007-8-4-r55

We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.

MeSH Terms (7)

Animals Chromosome Mapping Cloning, Molecular Genetic Linkage Genetic Markers Polymorphism, Single Nucleotide Zebrafish

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