Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures.

Pallan PS, Egli M
Nat Protoc. 2007 2 (3): 640-6

PMID: 17406625 · DOI:10.1038/nprot.2007.74

This protocol describes a simplified means of introducing an anomalously scattering atom into oligonucleotides by conventional solid-phase synthesis. Replacement of a nonbridging phosphate oxygen in the backbone with selenium is practically suitable for any nucleic acid. The resulting oligonucleotide P-diastereomers can be separated using anion exchange HPLC to yield diastereomerically pure phosphoroselenoates (PSes). The total time for the synthesis and ion-exchange HPLC separation of pure PSe is approximately 60 h.

MeSH Terms (7)

Chromatography, High Pressure Liquid Crystallography Molecular Structure Nucleic Acids Phosphorus Compounds Selenium Selenium Compounds

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