Yeast casein kinase I homologues: an essential gene pair.

Robinson LC, Hubbard EJ, Graves PR, DePaoli-Roach AA, Roach PJ, Kung C, Haas DW, Hagedorn CH, Goebl M, Culbertson MR
Proc Natl Acad Sci U S A. 1992 89 (1): 28-32

PMID: 1729698 · PMCID: PMC48168 · DOI:10.1073/pnas.89.1.28

We report the isolation of an essential pair of Saccharomyces cerevisiae genes that encode protein kinase homologues. The two genes were independently isolated as dosage-dependent suppressors. Increased dosage of YCK1 suppressed defects caused by reduced SNF1 protein kinase activity, and increased dosage of YCK2 relieved sensitivity of wild-type cells to salt stress. The two genes function identically in the two growth assays, and loss of function of either gene alone has no discernible effect on growth. However, loss of function of both genes results in inviability. The two predicted protein products share 77% overall amino acid identity and contain sequence elements conserved among protein kinases. Partial sequence obtained for rabbit casein kinase I shares 64% identity with the two yeast gene products. Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues.

MeSH Terms (16)

Amino Acid Sequence Casein Kinase I Casein Kinases Cloning, Molecular Gene Expression Genes, Fungal Genes, Suppressor Genetic Complementation Test Molecular Sequence Data Multigene Family Mutation Protein Kinases Restriction Mapping Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Alignment

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