High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells.

Raymond CS, Soriano P
PLoS One. 2007 2 (1): e162

PMID: 17225864 · PMCID: PMC1764711 · DOI:10.1371/journal.pone.0000162

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.

MeSH Terms (9)

Animals DNA Nucleotidyltransferases Embryo, Mammalian Genes, Reporter Genetic Engineering Integrases Mice Mice, Transgenic Recombination, Genetic

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