Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation.

Warnement MR, Faley SL, Wikswo JP, Rosenthal SJ
IEEE Trans Nanobioscience. 2006 5 (4): 268-72

PMID: 17181026 · DOI:10.1109/tnb.2006.886573

Antibody-conjugated quantum dots (QDs) have been used to map the expression dynamics of the cytokine receptor interleukin-2 receptor-alpha (IL-2Ralpha) following Jurkat T cell activation. Maximal receptor expression was observed 48 h after activation, followed by a sharp decrease consistent with IL-2R internalization subsequent to IL-2 engagement. Verification of T cell activation and specificity of QD labeling were demonstrated using fluorescence microscopy, ELISA, and FACS analyses. These antibody conjugates provide a versatile means to rapidly determine cell state and interrogate membrane associated proteins involved in cell signaling pathways. Ultimately, incorporation with a microfluidic platform capable of simultaneously monitoring several cell signaling pathways will aid in toxin detection and discrimination.

MeSH Terms (9)

Cytokines Humans Jurkat Cells Lymphocyte Activation Microscopy, Fluorescence Molecular Probe Techniques Quantum Dots Receptors, Cytokine T-Lymphocytes

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