A reliable method for northern blot analysis using synthetic oligonucleotide probes.

Henderson GS, Conary JT, Davidson JM, Stewart SJ, House FS, McCurley TL
Biotechniques. 1991 10 (2): 190-7

PMID: 1711871

We have developed a method for using short (30-42 base pair) synthetic oligonucleotide DNA probes in Northern blot assays. The method involves labeling the probes to high specific activity, very stringent hybridization and wash conditions, and the presence of several inhibitors of nonspecific binding in the hybridization buffer. We have tested this method with several probes obtained from local and commercial sources. The results with every probe used were high signal-to-noise ratios in an exposure time range of 30 min to 7 days.

MeSH Terms (12)

Animals Blotting, Northern Cell Line DNA Probes Humans Lymphokines Mice Mice, Inbred CBA Oligonucleotide Probes Prealbumin RNA Schistosomiasis mansoni

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