Selective restriction of Nef-defective human immunodeficiency virus type 1 by a proteasome-dependent mechanism.

Qi M, Aiken C
J Virol. 2007 81 (3): 1534-6

PMID: 17108041 · PMCID: PMC1797497 · DOI:10.1128/JVI.02099-06

The Nef protein enhances human immunodeficiency virus type 1 (HIV-1) infectivity by facilitating an early postentry step in the virus life cycle. We report here that the addition of MG132 or lactacystin, each a specific inhibitor of cellular proteasome activity, preferentially enhances cellular permissiveness to infection by Nef-defective versus wild-type HIV-1. Pseudotyping by the glycoprotein of vesicular stomatitis virus rendered Nef-defective HIV-1 particles minimally responsive to the enhancing effects of proteasome inhibitors. These results suggest that Nef enhances the infectivity of HIV-1 particles by reducing their susceptibility to proteasomal degradation in target cells.

MeSH Terms (6)

Cysteine Endopeptidases Gene Products, nef HIV-1 Humans nef Gene Products, Human Immunodeficiency Virus Proteasome Endopeptidase Complex

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