The transcriptional activity of CITED1 is regulated by phosphorylation in a cell cycle-dependent manner.

Shi G, Boyle SC, Sparrow DB, Dunwoodie SL, Shioda T, de Caestecker MP
J Biol Chem. 2006 281 (37): 27426-35

PMID: 16864582 · DOI:10.1074/jbc.M602631200

CITED1 is the founding member of the CITED family of cofactors that are involved in regulating a wide variety of CBP/p300-dependent transcriptional responses. In the present study, we show that the phosphorylation status of CITED1 changes during the cell cycle and affects its transcriptional cofactor activity. Tryptic mapping and mutagenesis studies identified five phosphorylated serine residues in CITED1. Phosphorylation of these residues did not affect CRM1-dependent nuclear export, but did decrease CITED1 binding to p300 and inhibited CITED1-dependent transactivation of Smad4 and p300. These results suggest that CITED1 functions as a cell cycle-dependent transcriptional cofactor whose activity is regulated by phosphorylation.

MeSH Terms (16)

Apoptosis Regulatory Proteins Cell Cycle Cell Line, Tumor Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation Humans Mutagenesis Nuclear Proteins p300-CBP Transcription Factors Phosphorylation Smad4 Protein Subcellular Fractions Trans-Activators Transcriptional Activation Transcription Factors Trypsin

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