PARP regulates TGF-beta receptor type II expression in estrogen receptor-positive breast cancer cell lines.

Sterling JA, Wu L, Banerji SS
Anticancer Res. 2006 26 (3A): 1893-901

PMID: 16827122

BACKGROUND - Expression of the tumor suppressor gene, transforming growth factor beta receptor type II (TbetaRII) is often reduced in estrogen receptor-positive breast cancer cells leading to uninhibited tumor cell growth. A clear understanding of its regulation is necessary to identify potential mechanisms to re-express this tumor suppressor gene.

MATERIALS AND METHODS - The regulation of TbetaRII expression was studied by utilizing 5'promoter deletion constructs, followed by EMSA analyses. The resulting binding protein was affinity purified and identified by mass spectrophotometry.

RESULTS - An inverted CCAAT box centered at -79 was found to be essential for TbetaRII promoter activity. Purification of the protein binding to this region and subsequent mass spectrophotometric analysis identified the binding protein as poly(ADP-ribose)polymerase I (PARP). ChIP assays verified that PARP interacted with the TbetaRII promoter in vivo.

CONCLUSION - The present study demonstrated that PARP is important for TbetaRII expression in estrogen receptor-positive breast cancer cell lines.

MeSH Terms (14)

Breast Neoplasms CCAAT-Binding Factor Cell Line, Tumor Gene Expression Regulation, Neoplastic Humans Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Receptor, Transforming Growth Factor-beta Type II Receptors, Estrogen Receptors, Transforming Growth Factor beta Transcription Factors Transfection

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