A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme.

Luka Z, Capdevila A, Mato JM, Wagner C
Transgenic Res. 2006 15 (3): 393-7

PMID: 16779654 · PMCID: PMC2792375 · DOI:10.1007/s11248-006-0008-1

Three human cases having mutations in the glycine N-methyltransferase (GNMT) gene have been reported. This enzyme transfers a methyl group from S-adenosylmethionine (SAM) to glycine to form S-adenosylhomocysteine (SAH) and N-methylglycine (sarcosine) and is believed to be involved in the regulation of methylation. All three cases have mild liver disease but they seem otherwise unaffected. To study this further, gnmt deficient mice were generated for the first time. This resulted in the complete absence of GNMT protein and its activity in livers of homozygous mice. Compared to WT animals the absence of GNMT resulted in up to a 7-fold increase of free methionine and up to a 35-fold increase of SAM. The amount of SAH was significantly decreased (3 fold) in the homozygotes compared to WT. The ratio of SAM/SAH increased from 3 in WT to 300 in livers of homozygous transgenic mice. This suggests a possible significant change in methylation in the liver and other organs where GNMT is expressed.

MeSH Terms (15)

Animals Disease Models, Animal DNA Methylation Genetic Techniques Glycine N-Methyltransferase Homozygote Humans Liver Metabolism, Inborn Errors Mice Mice, Knockout Mice, Transgenic Mutation Plasmids Promoter Regions, Genetic

Connections (1)

This publication is referenced by other Labnodes entities: