T-reg mediated suppression of the allograft response in the draining lymph node.

Lee MK, Moore DJ, Chiaccio M, Lian MM, Deng S, Mohiuddin M, Huang X, Koeberlein B, Zakheim A, Porrett PM, Barker CF, Caton AJ, Markmann JF
Transplantation. 2006 81 (7): 1063-6

PMID: 16612285 · DOI:10.1097/01.tp.0000168365.80771.5a

We previously demonstrated that T-regs inhibit proliferation of graft-reactive T cells in the draining lymph node (DLN), suggesting that this site may be important for regulation. TCR transgenic mice (TS1) specific for viral hemagglutinin (HA) provided antigen-specific T cells for adoptive transfer into syngeneic Balb/c hosts bearing HA+ skin grafts. T-regs were obtained from (TS1xHA28)F1 mice known to have an expanded population of HA-specific T-regs. To determine whether the lymph node is an independent site of suppression, we developed a model in which donor antigen that migrates from the allograft to the DLN drives T-cell activation after graft removal. T-regs that did not encounter the allograft itself remained able to inhibit graft antigen-specific T-cell proliferation in the DLN. Alloantigen-induced regulation can occur in the absence of the graft. This finding identifies the DLN as a potentially critical site of regulation in the early posttransplant period.

MeSH Terms (11)

Animals Immunosuppression Lymph Nodes Mice Mice, Inbred BALB C Mice, Transgenic Receptors, Interleukin-2 Skin Transplantation T-Lymphocytes, Regulatory Transplantation, Homologous Transplantation Tolerance

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