Assessment of Rab11-FIP2 interacting proteins in vitro.

Ducharme NA, Jin M, Lapierre LA, Goldenring JR
Methods Enzymol. 2005 403: 706-15

PMID: 16473632 · DOI:10.1016/S0076-6879(05)03061-2

Members of the Rab family of small GTPases are involved in multiple trafficking events in both endocytotic and biosynthetic pathways. To understand more fully the regulation of these events, a concerted effort is underway to ascertain the binding partners and regulators of Rabs. Here, we describe methods to assess binding of Rab11a with Rab11-FIP2 and other Rab11-FIPs utilizing a modified far-Western approach. We then broaden this application to assess binding of Rab11-FIP2 with myosin Vb and homodimerization of Rab11-FIP2.

MeSH Terms (11)

Animals Carrier Proteins Dogs Electrophoresis, Polyacrylamide Gel Membrane Proteins Myosin Heavy Chains Myosins Myosin Type V Protein Binding rab GTP-Binding Proteins Recombinant Proteins

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