Maturation of the viral core enhances the fusion of HIV-1 particles with primary human T cells and monocyte-derived macrophages.

Jiang J, Aiken C
Virology. 2006 346 (2): 460-8

PMID: 16375941 · DOI:10.1016/j.virol.2005.11.008

HIV-1 infection requires fusion of viral and cellular membranes in a reaction catalyzed by the viral envelope proteins gp120 and gp41. We recently reported that efficient HIV-1 particle fusion with target cells is linked to maturation of the viral core by an activity of the gp41 cytoplasmic domain. Here, we show that maturation enhances the fusion of a variety of recombinant viruses bearing primary and laboratory-adapted Env proteins with primary human CD4+ T cells. Overall, HIV-1 fusion was more dependent on maturation for viruses bearing X4-tropic envelope proteins than for R5-tropic viruses. Fusion of HIV-1 with monocyte-derived macrophages was also dependent on particle maturation. We conclude that the ability to couple fusion to particle maturation is a common feature of HIV-1 Env proteins and may play an important role during HIV-1 replication in vivo.

MeSH Terms (11)

CD4-Positive T-Lymphocytes Cell Line HIV-1 HIV Envelope Protein gp41 Humans Macrophages Membrane Fusion Receptors, CCR5 Receptors, CXCR4 Receptors, HIV Virus Replication

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